The Institute for Neurodegenerative Diseases at the University of California San Francisco
Bioanalytical/HPLC-Mass Spectrometry Researcher
Staff Research Associate II
Staff Research Associate III
Postdoctoral Scholar
INSTITUTE FOR NEURODEGENERATIVE DISEASES
Bioanalytical/HPLC-Mass Spectrometry Researcher
The Institute for Neurodegenerative Diseases (IND) at the University of California, San Francisco (UCSF) is seeking an outstanding and accomplished scientist with experience in Bioanalytical/HPLC-Mass Spectrometry. S/he would immediately contribute to ongoing drug discovery programs in neurodegenerative diseases, including Creutzfeldt-Jakob disease (CJD), Alzheimer's disease (AD), Frontotemporal dementia (FTD), and Parkinson's disease (PD). The IND is building a highly focused and multidisciplinary team to discover the causes of disease, and develop effective pharmacologic treatments, cures and predictive diagnostic markers for these diseases. The IND is directed by Nobel Laureate Dr. Stanley Prusiner and is committed to discovering the causes and developing treatments, cures and diagnostics for neurodegenerative diseases. Drug discovery research and development at the IND is headed by Dr. Michael Silber. The IND is comprised of scientists and faculty across multiple UC campuses.
The incumbent will be an outstanding B.S., M.S., or Ph.D. level Bioanalytical/HPLC-Mass Spectrometry researcher with significant experience applying state-of-the-art liquid chromatography-mass spectrometry (LC-MS) and matrix assisted laser desorption ionization time of flight (MALDI-TOF) MS to support drug discovery research in the small molecule, peptide and protein area and report to Dr. Silber. S/he will immediately be responsible, hands-on, for LC-MS assay development and sample analysis currently using a Sciex API-4000, sample preparation, pharmacokinetic study design and execution, formulation evaluation and preparation, and with the pharmacology team, design and execution of studies in animals using appropriate disease models to identify relationships between efficacy and drug exposure especially in the brain. The candidate will also be responsible in the future for the application and use of a MALDI-TOF MS for the analysis of peptides and proteins in biological matrices.
The candidate will use broad scientific knowledge to contribute to sample preparation, LC-MS and MALDI-TOF MS methods development, sample analysis, and data analysis. S/he will use her/his technical expertise to evaluate, select, and apply novel methodologies using state-of-the-art LC-MS and MALDI-TOF MS instrumentation, especially the Sciex API-4000 now. S/he will work independently to design and conduct bioanalytical, pharmacokinetic and structure-based experiments, and document, interpret, communicate, apply and publish results relating to drugs targeting the brain for the treatment of neurodegenerative diseases.
This is a remarkable opportunity for an accomplished bioanalytical/LC-MS/MALDI-TOF MS researcher to work in a unique interdisciplinary environment in a "100% hands-on" position where collaborations are highly encouraged and many supporting core activities are readily available. All candidates with exceptional records of accomplishment in the LC-MS and MALDI-TOF MS areas as applied to a myriad of applications in drug discovery research will be considered. Relevant industrial (Pharma/Biotech/startup) experience preferable, but candidates with equivalent experience in an academic or nonprofit setting will be considered. The ideal candidate will have knowledge, experience, and a proven track record in the field of bioanalytical chemistry, LC-MS, MALDI-TOF MS, and pharmacokinetics, demonstrated by accomplishments, including publications in peer-reviewed journals.
QUALIFICATIONS:
- B.S., M.S., or Ph.D. in Biological Sciences, Analytical Chemistry, Bioanalytical Chemistry, Biochemistry, or a related science.
- Demonstrated experience in applying LC-MS to drug discovery studies and biological sample handling and processing in a drug discovery setting, as well as experience determining peptide and protein structure required.
- For B.S. or M.S. levels candidates, not less than 7 years of directly relevant hands-on experience working independently in LC-MS and MALDI-TOF MS methods development, evaluation, optimization, validation, and application to drug discovery. For candidates with a Ph.D., not less than 3 years of experience.
- Actual hands-on experience, responsibility and accountability for operating a triple quadrupole MS like a Sciex API-4000 and a MALDI-TOF MS required.
- Expertise in bioanalytical, pharmacokinetic, and pharmacology experimental design and critical analytical skills, including organic chemistry, chromatographic separations, mass spectrometry, bioanalytical extraction techniques, and basic pharmacokinetic analysis.
- Expertise with various biological matrices, including brain, liver, kidney.
- Understanding and use of relational database concepts and hands-on experience with data input, querying, report generation, experimental design and analytical skills.
- Expertise with MALDI-TOF MS in peptide and protein sequencing applications in biology and drug discovery.
- Thorough understanding of generating and manipulating databases, concepts and hands-on experience with data input, querying, report generation, and programming.
- Expertise in the use of key laboratory equipment used in support of LC/MS-MS experimental work.
- Strong computer skills and proficiency with quantitative software and programming.
- Excellent oral and written communication and organizational and record keeping skills, and the ability to work as an effective and efficient team member with colleagues and peers within and outside of the IND.
- Potential to contribute to manuscripts reporting research results.
Applicants should submit a cover letter and resume to ind@ind.ucsf.edu. Refer to “Bioanalytical/Pharmacokinetic Chemist” when applying for this position.
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INSTITUTE FOR NEURODEGENERATIVE DISEASES
The Institute for Neurodegenerative Diseases (IND) at the University of California, San Francisco (UCSF) is seeking a Staff Research Assistant II to join the IND’s high-throughput screening (HTS) team, in support of our expanding Drug Discovery programs in neurodegenerative diseases, including Prion diseases like Creutzfeldt-Jakob disease (CJD), Alzheimer’s Disease (AD), Frontotemporal Dementia (FTD), and Parkinson’s disease (PD). The IND is directed by Nobel Laureate Dr. Stanley Prusiner. Dr. Michael Silber heads the IND Drug Discovery R & D efforts, including the screening efforts, and reports to Dr. Prusiner.
Staff Research Associate II
This position will contribute, as part of the HTS team, to the screening of FDA-approved and foreign approved drugs (FAD) and chemical compounds in diverse chemical libraries to identify "hits" leading to the discovery and development of treatments and cures for a number of neurodegenerative diseases.
The incumbent will have hands-on experience screening of drugs and compounds using cell-, biochemical-, and other screening based assays, and for converting assays to a HTS format. The successful candidate will join a HTS team, which is part of an overall multidisciplinary Drug Discovery research effort whose research interests span many fields of scientific research and report to the Team Leader. The goal of the team is to identify effective therapeutics against neurodegenerative diseases, including prion diseases like CJD, AD, FTD, PD and other neurodegenerative diseases.
This position will be located at our South San Francisco location. At this time, there are no UCSF shuttles offering service to our South San Francisco location. Employees will be responsible for their own transportation.
REQUIRED QUALIFICATIONS, AND SPECIFIC JOB FOCUS, RESPONSIBILITIES, and EXPECTATIONS:
- B.S. in Biology, Cell Biology, Biochemistry, Immunology or related science with at least 4+ years of applied experience in the areas indicated below, or a Master's of Science degree with at least 1-2 years of experience.
- Technical and scientific knowledge and experience with cell based and biochemical assays and HTS.
- Technical and scientific experience in mammalian cell culture and sterile technique.
- Technical and scientific knowledge and experience with various techniques, including biochemical protein quantification, protein purification, cell and tissue fractionation, Western blots, ELISA, cell fractionation, immunocytochemistry and immunofluorescence.
- Experience handling, preparing and tracking chemical compounds.
- Technical and scientific knowledge in data analysis and QC.
- Understanding and use of relational database concepts and hands-on experience with data input, querying, report generation, experimental design and analytical skills.
- 90% of the time will be devoted to hands-on lab work, especially bench work, along with appropriate planning, data analysis, documentation and supply management required to complete all aspects the experimental work and will specifically include the following technical and scientific activities:
- use, maintenance, and updating of laboratory notebooks.
- HTS using cell based and biochemical assays.
- mammalian cell culture and sterile technique.
- basic molecular biology, biochemistry, ELISA, Western blot, pipetting, robotics, and handling of plates.
- use and basic programming of automation systems.
- use and troubleshooting of plate readers, light and fluorescence microscopes.
- use of computers and all appropriate software, along with basic programming skills.
- metadata preparation, data acquisition, management and analysis.
- Excellent time management skills.
- Excellent oral and written communication skills, organizational and record keeping skills.
- Ability to work as an effective and efficient team member, and to participate in team meetings and planning.
- Enthusiasm, positive outlook, collegiality and scientific curiosity.
Interested applicants should submit curriculum vitae with a list of publications, a brief statement of research accomplishments and interests and contact information for three to four professional references.
Applicants should submit a cover letter and resume to drugdiscovery@ind.ucsf.edu.
Refer to"Drug Discovery" when applying for this position.
UCSF is an equal opportunity employer. Diversity is encouraged. Veterans, minorities and women are encouraged to apply.
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INSTITUTE FOR NEURODEGENERATIVE DISEASES
The Institute for Neurodegenerative Diseases (IND) at the University of California, San Francisco (UCSF) is seeking a Staff Research Assistant III to join the IND's high-throughput screening (HTS) team, in support of our expanding Drug Discovery programs in neurodegenerative diseases, including Prion diseases like Creutzfeldt-Jakob disease (CJD), Alzheimer's Disease (AD), Frontotemporal Dementia (FTD), and Parkinson's disease (PD). The IND is directed by Nobel Laureate Dr. Stanley Prusiner. Dr. Michael Silber heads the IND Drug Discovery R & D efforts, including the screening efforts, and reports to Dr. Prusiner.
This position will be responsible for screening of FDA-approved and foreign approved drugs (FAD) and chemical compounds in diverse chemical libraries to identify "hits" leading to the discovery and development of treatments and cures for a number of neurodegenerative diseases.
The incumbent will have significant knowledge, and expert level technical and scientific experience and will report to the HTS team leader. S/he will be responsible and accountable for the development, validation, and hands-on screening of drugs/compounds using cell-, biochemical-, and other screening based assays, and for converting assays to a HTS format.
The successful candidate will join a HTS team, which is part of an overall multidisciplinary Drug Discovery research effort whose research interests span many fields of scientific research. The goal of the team is to identify effective therapeutics against neurodegenerative diseases, including prion diseases like CJD, AD, FTD, PD and other neurodegenerative diseases.
This position will be located at our South San Francisco location. At this time, there are no UCSF shuttles offering service to our South San Francisco location. Employees will be responsible for their own transportation.
REQUIRED QUALIFICATIONS, AND SPECIFIC JOB FOCUS, RESPONSIBILITIES, and EXPECTATIONS:
- B.S. in Biology, Cell Biology, Biochemistry, Immunology or related science with at least 6+ years of applied experience in the areas indicated below, or a Master's of Science degree with at least 3+ year of experience.
- Expert technical and scientific knowledge and experience with HTS using cell based and biochemical assays; demonstrated contribution to the successful completion of a HTS campaign.
- Expert technical and scientific experience in mammalian cell culture and sterile technique.
- Expert technical and scientific knowledge and experience in biochemical protein quantification and characterization techniques (protein purification, cell and tissue fractionation, Western blots and ELISA), cell fractionation, immunocytochemistry and immunofluorescence based techniques.
- Expert technical and scientific knowledge and experience handling, preparing and tracking chemical compounds in 96 or 384 well format.
- Expert technical and scientific knowledge in data analysis and QC of large and complex data sets.
- High level understanding and use of relational database concepts and hands-on experience with data input, querying, and report generation; programming skills and strong experimental design and analytical skills.
- 80-90% of the time will be devoted to hands-on lab work, especially bench work, along with appropriate planning, data analysis, documentation and supply management required to complete all aspects the experimental work at high levels of expertise and will specifically include the following technical and scientific activities:
- use, maintenance, and timely updating of laboratory notebooks.
- HTS using cell based and biochemical assays; hands-on contributions to the successful completion of HTS campaigns.
- Mammalian cell culture and sterile technique.
- basic molecular biology (cloning, PCR, primer design) and biochemistry (protein purification, cell and tissue fractionation, western blots and ELISA) techniques, pipetting, robotics, and handling of plates, and handling, preparing and tracking chemical compounds in 96 or 384 well format.
- use and programming of automation systems.
- use and troubleshooting of plate readers; experience with light and fluorescence microscopes.
- use of computers and all appropriate software, along with programming skills.
- metadata preparation, data acquisition, management and analysis.
- Excellent time management skills.
- Excellent oral and written communication skills, organizational and record keeping skills.
- Ability to work as an effective and efficient team member, and to participate in team meetings and planning.
- Enthusiasm, positive outlook, collegiality and scientific curiosity.
Interested applicants should submit curriculum vitae with a list of publications, a brief statement of research accomplishments and interests and contact information for three to four professional references.
Applicants should submit a cover letter and resume to drugdiscovery@ind.ucsf.edu. Refer to "Drug Discovery SRA" when applying for this position.
UCSF is an equal opportunity employer. Diversity is encouraged. Veterans, minorities and women are encouraged to apply.
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The Prusiner Laboratory is offering postdoctoral positions in the field of prion biology to highly qualified and motivated postgraduates. To be eligible, candidates must have attained a Ph.D. degree in biochemistry, biophysics, cell biology, genetics, molecular biology or neuroscience, have a passion for research and a strong desire to discover the causes and cures for neurodegenerative diseases.
Interested applicants should submit curriculum vitae with a list of publications, a brief statement of research accomplishments and interests and contact information for three professional references.
Email to: ind@ind.ucsf.edu
Refer to "Postdoctoral Scholar" when applying for this position.
The University of California, San Francisco is an equal opportunity employer.
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